Hi,
I'm just curious if I am able to obtain good quality data of 600 bp read length using MiSeq v3 600 cycle kit.
I know the read quality goes down with longer read length.
Has anyone tried using this kit to get longer read lengths?
Other question is, is there any good NGS technique / platform that yields long read lengths (~500 bp>) ?
Thanks for all the help
Yes. MiSeq kits can be run either in PE or SE mode. SE mode yields half the reads, but twice the length. As you say the read quality will be quite poor at the end of the reads. Even at 400bp the qualities weren't great.
For really long reads look at PacBio and Oxford Nanopore. Both can routinely generate ~10kbp reads and exceptionally 100kbp. These technologies are very different as they generate far fewer reads and the reads are of variable length.
There are many centres around which provide PacBio sequencing as a service, but not Oxford Nanopore. That's not an issue as the minION instrument is only ~£1000 and is the size of a stapler. Groups tend to just buy them.