Donghyun Dave Lee

5 Questions 2 Answers 0 Followers

Questions related from Donghyun Dave Lee

Using RE that creates blunt end for 4C (Circularized Chromosome Conformation capture)?

Has anyone tried using RE that creates blunt end (e.g. PvuII) for primary RE in 4C (Circularized Chromosome Conformation Capture) Assay? My bait ("viewpoint") region has very limited RE...

29 November 2016 5,734 6 View

Can I obtain 1 x 600 bp read length on MiSeq v3 600 Cycle kit?

Hi, I'm just curious if I am able to obtain good quality data of 600 bp read length using MiSeq v3 600 cycle kit. I know the read quality goes down with longer read length. Has anyone tried using...

04 November 2016 1,560 1 View

ChIP on suspension cells?

Is there any difference in performing ChIP on suspension cells vs. adherent cells? I'm currently using Activemotif ChIP-IT Express Enzymatic Kit

26 July 2016 8,457 3 View

Any advice on ARMS PCR Primer design?

Hi all, I'm trying to perform ARMS PCR to see if certain cell lines that I'm interested in show heterozygosity for SNP rs2736098 (in exon 2 of TERT gene) This is my first time doing ARMS PCR and...

08 June 2015 1,530 7 View

Post-midiprep plasmid sequencing?

I've successfully cloned my fragments into pGL3 (Luciferase) vector, and I want to see if the bands that I see on my agarose gel are correct by sequencing. So I was just wondering what the ideal...

05 November 2013 3,048 0 View