Can I measure the amount of AAV capsid with Bradford assay?

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Shamsher S. Kanwar

You may assay the protein content of AAV capsid, but it depends on the visual pellet obtained after a density gradient ultracentrifugation run.

Jakub Knurek

Hi Byoungchan Park,

Bradford Assay is effective for most proteins, but there are other, more suitable methods for accurately quantifying the amount of AAV capsid particles. The presence of DNA in the AAV capsid may lead to inaccurate protein concentration measurements. I prefer ELISA-based methods using antibodies specific to the capsid protein, which can provide quantitative measurements of AAV particles. You can also use other methods described in the literature:

Article Comparison of Analytical Techniques to Quantitate the Capsid...

Article Characterization of Adeno-Associated Virus Capsid Proteins w...

Article Quantification of Adeno-Associated Virus Particles and Empty...

Article Accurate Quantification and Characterization of Adeno-Associ...

https://www.nature.com/articles/s41598-021-82599-1

Good luck