I understand that Adenovirus is co-infected during AAV TCID50 to provide helper components for AAV replication.
But why should AAV be replicated?
Can't you infect cells only with AAV and calculate copy number inside the target cell?
TCID50 measures functional, infectious virus particles. Simply measuring the copy number inside the cells doesn't distinguish between intact, functional viral genome and degraded viral material. TCID50 helps assess the proportion of functional AAV particles in the sample.
Adenovirus co-infection provides helper functions, allowing AAV to replicate within the cell. This replication cycle enables the TCID50 assay to indirectly detect functional AAV by observing its ability to take advantage of the helper virus and propagate.
In summary, AAV replication is necessary for TCID50 because it allows researchers to indirectly assess the presence of functional AAV particles through their ability to replicate in the presence of helper adenovirus. Measuring copy number alone doesn't guarantee infectivity.
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