Can I insert sequence (200bp-800bp) in pUC57 plasmid for PCR and qPCR applications??

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Laurence Stuart Dawkins-Hall

In essence yes you can
However, for qPCR (not PCR) keep in mind that you can only amplify fragments of 100bp to ensure your primer efficiency is > 90% (ideally >95%)

Hend Altaib

I think it is yes. I have tried a similar experiment but, with another pUC and I also agree with Professor Laurence