Luis A. Salcedo Mejía

4 Questions 3 Answers 0 Followers

Questions related from Luis A. Salcedo Mejía

Can I insert sequence (200bp-800bp) in pUC57 plasmid for PCR and qPCR applications??

I have to PCR assays. I want to prepare positive controls with DNA synthetic. But, I'm not sure use pUC57 plasmid. My PCR product are between 200 -800 bp. What other considerations should I have?

03 February 2020 4,724 2 View

The incubation with isopropanol to -20°C overnight increase the ARN recuperation?

Hi all, I did read few papers that write protocols with overnight incubation with isopropanol for increase RNA recuperation, but the handbook (f.e. qiazol) does not mention it, only an incubation...

27 February 2017 4,359 6 View

What statistical technique I use and how I use it to analyze a comparison between a native strain and control strain for the solubilization ofcopper?

evaluated the bioleaching of a native strain compared with a control strain, have variables (cell concentration,% solubilization of copper, time) and the taking of the data is that starting from a...

14 April 2016 8,646 1 View

When using heap leach or stirred tank bioreactors, does it depend on the mineral to use?

There are many processes / patents using bioleaching of sulphide ores or oxidized copper / gold, using both batteries as bioreactors, but this will depend on that? The percentage of precious metal...

26 June 2014 8,814 3 View