Luis A. Salcedo Mejía

8 Questions 8 Answers 0 Followers

Questions related from Luis A. Salcedo Mejía

Can I insert sequence (200bp-800bp) in pUC57 plasmid for PCR and qPCR applications??

I have to PCR assays. I want to prepare positive controls with DNA synthetic. But, I'm not sure use pUC57 plasmid. My PCR product are between 200 -800 bp. What other considerations should I have?

03 February 2020 4,838 2 View

The incubation with isopropanol to -20°C overnight increase the ARN recuperation?

Hi all, I did read few papers that write protocols with overnight incubation with isopropanol for increase RNA recuperation, but the handbook (f.e. qiazol) does not mention it, only an incubation...

27 February 2017 4,473 6 View

What statistical technique I use and how I use it to analyze a comparison between a native strain and control strain for the solubilization ofcopper?

evaluated the bioleaching of a native strain compared with a control strain, have variables (cell concentration,% solubilization of copper, time) and the taking of the data is that starting from a...

14 April 2016 8,751 1 View

Is there any tutorial to assemble and analyze sequences from sanger sequencing?

I recently sent samples to be sequenced in Macrogen-Korea, but do not know much assemble secuenciasy analyze the quality of sequencing. So I want to learn.

17 March 2016 412 6 View

Is it possible to perform MPN for acidophiles iron-oxidizing in microplate-96?

I'm performing an experiment of resistance to arsenic than 10 microbial consortia acidophilus, and I want to determine the number of viable cells present.For my budget I can not do the experiment...

24 June 2015 8,198 7 View

Is the bacterial leaching a (non-polluting) ecofriendly technology? If so why?

I have reviewed several papers in which this phrase is mentioned but not described why it is.

16 February 2015 2,260 6 View

When using heap leach or stirred tank bioreactors, does it depend on the mineral to use?

There are many processes / patents using bioleaching of sulphide ores or oxidized copper / gold, using both batteries as bioreactors, but this will depend on that? The percentage of precious metal...

26 June 2014 8,908 3 View

Why can't Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans grow successfully in inorganic solid medium (as 9K, TK, etc.)?

In liquid cultures with inorganic ferrous sulfate At. ferrooxidans and L. ferrooxidans grow fine but when trying to isolate on solid medium which is growing successfully At. ferrooxidans and L....

11 April 2014 1,076 3 View