I ran a gel electrophoresis at 1% agarose for genomic DNA of 2 endemic plants. I did a duplicate. The first two lanes on the left are the first plant. The 2 lanes on the right are the second plant. I ran the gel at 115 Volts for 40 minutes.
What were you expecting to find? How large is the genomic DNA? Genomic DNA is usually the largest band in any lane on a gel, but without a standard or ladder of some size, we can't even tell the relative size of each band.
well, i was told that i should have one band instead of that many.
if i had a smear i would i have thought maybe i need an RNAse treatment. I did this gel in order to know whether my extraction was good or not. I dont know whether it is DNA from the plants or is it because of contamination that am having this gel.
Ok i will try to do the extraction again with fresh young leaves this time.
There seem to be a problem with your mode of extraction. If the two lanes on the left are of the same plant, i expect them to have the same bands which is not the case. For this reason, i would discard these results, look at the protocol and trouble shooting of the protocol and then repeat again, more keenly.
I did the whole thing again today. This time i cleaned everything with alcohol. I'll wait and see next week.
I agree with Harry Caufield. What do you expect? The concentration of agarose gel is dependent from the expected molecular size of your target. I suggest you to run a ladder having a molecular range of the expected DNA.
Hey guys i need some advice. Well am extracting DNA from an endemic plant leaf. When i spin at 8000rpm for 30 minutes with ice cold isopropanol, i get flaky white deposit. Well i suppose it is DNA, but is it possible to get so much DNA?
Ok sir , this is really helpful. I will do an RNAse treatment. Its for my final year project. Thanks
It worked this time. I used fresh young leaves and cleaned the motar every time with alcohol. I suppose the result for the first gel was really contamination.
My paper has been published and its available. Check it out. Thanks for your help
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