I am dealing with plant DNA. I have already tried a protocol which has phenol:chloroform:isoamyl. What I did was to use 35 microlitres of DNA+ 65 microlitres of sterile water. Then I added 1 microlitre of RNAse A. I placed the eppendorf in a waterbath at 37 degrees for 1 hour. I used sodium acetate 3M. I think the rest of the protocol you already know.
I then did a gel electrophoresis of 1% but nothing appeared which meant my DNA was degraded.
The weird thing is during, the spinning process of isopropanol I saw a ppt of DNA at the bottom of my eppendorf even after washing with alcohol 70%. I removed the alcohol, dried the pellet at 45 degrees and added 40 microlitres of sterile water to dissolve the white ppt of DNA.
Can anyone of you help me?
Change out your water to DNase/RNase free. Also, when you freeze to precipitate your DNA, do so overnight. It may be that your DNA has been degraded or you just got extremely low yield.
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