Can I get some suggestions to choose a Multi probe RNase Protection Assay (RPA - non radio active) kit?

24 April 2020 0 4K Report

I am going to check oxytocin receptor, Cn43 and some other genes expression in mice uterus during labor (including my gene of interest). Thinking to use the RPA method to show a set of highly regulated genes during labor in a single blotting image panel.

Is this RPA method better than northern and RT-qPCR in this context?

Can someone share their experience/advice about Multi probe RPA ? (mainly probe design using biotin labeling, kit names etc., )

Kits I read about -> RPA III™ Ribonuclease Protection Assay Kit From Ambion (now Applied Biosciences) with RiboQuant™ Multi-Probe Template Sets From BD Biosciences

Thanks in Advance!

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