I intend to co-culture BMDC with irradiated B16 cells as a vaccine fto prevent B16 tumorigenesis. I need to immunize four times,but it is very inconvenient to treat tumor cells with radiation each time. Can I irradiate a sufficient amount of tumor cells with the corresponding dose at one time, and then freeze them in batches, and then thaw them when I plan to use?
thank you very much for your help, this article provides me much useful information.But I want to preserve the irradiated tumor cells in liquid nitrogen and thaw them when i need.by that i mean these irradiated tumor cells only freeze and thaw once instead of freezing and thawing them repeatedly. I wonder if this process will affect subsequent experiments. could you give me some advice?@ Saleh Alkarim
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