Why are there two reduction peaks in my cyclic voltammogram?

When I measured the cyclic voltammogram of palladium in the pbs solution, two reduction peaks appeared. This did not match the literature I saw. Why? And how can I find the possible reaction...

20 February 2021 2,486 3 View

Can we assume the water bottom reflectacne does not change and remain at a certain value?

Hi guys, I am trying to understand if the water bottom type does not change, its reflectance at a certain wavelength changes or not?

15 October 2020 6,297 3 View

Does grain size have a correlation with their colours?

Among gravel, sands, silt and clay, these grains have different sizes. Except their original materials, do they appear different colours due to different abilities of reflection?

04 November 2019 1,279 5 View

Is it possible to convert the DN value of a Sentinel 2 imagery to a matrix?

Hi, I would like to process my data in Matlab (I have to process them in Matlab). Is it possible to convert the DN value (band 2) of a sentinel 2 imagery to a matrix, which means each cell...

16 September 2019 6,267 3 View

How plot the scatters and curves in the same graph in Matlab?

Hi guys. I wanna combine the scatters and curves in the same graph. However, I used spline function in curves. The graph twisted a lot. The expected graph is the...

28 August 2019 6,684 1 View

How to calculate intersystem crossing rate from Dalton?

Dear all, I'd like to calculate the intersystem crossing rate using Dalton package, for example, from S1 to T1. Now I can only get the spin orbit coupling matrix element (SOCME), but don't know...

21 August 2019 6,595 4 View

Regression assumption tests. Are these residuals heteroscadastic or homoscadastic?

Can these residuals be regarded heteroscadastic? I have tested the residuals are normally distributed.

23 May 2019 5,173 4 View

What is the appropriate OD/wavelength (spectrophotometer) for measuring bacterial growth in broth?

Hi all, I am working on growing actinomycetes in broth and to measure its turbidity in indicating the presence of growth. Why are we measuring optical density of bacteria at 600nm in general?...

18 September 2018 1,697 7 View

How to count cells in FACS

Hi everyone! I'm new to the world of flow cytometry, I want to count the T cells in the rat liver, After getting the monocytes from the liver, I stained the CD3, then first I gate the monocytes...

26 July 2017 2,833 3 View

What is the best culture medium to analyze total anaerobe and total facultivate bacteria?

04 May 2017 1,183 4 View

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02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View