Can I express protein in E.coli ?

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Get more than 80% - 90% of transcription efficiency

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I Want a plagiarism software for my personal use , please share where it available (Link) , price detais?

My own question

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Is there simple and efficient way or any parametrization method to estimate glacier basal velocity for a valley glacier?

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Poly Ethylene Glycol 6000 Measurement?

How to measure/convert the Mpa (PEG-6000) to Grams? for example -2.0Mpa = how many grams?

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Why the mass conservation ice thickness model approach is superior to the other ice thickness model approaches like GlabTop or velocity-slope?

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Adhesive for contact lenses ?

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In gabbro rock LREE shows enriched pattern pls explain about magma emplacement and tectonic setting of this rock ?

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How to find the relative importance of input variable in Machine Learning models?

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How can we now if the promotor in the vector is well?

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What is the acceptable p-value cutoff for GO enrichment analysis ?

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Transfection in HEK293T cells?

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How to retain the a GFP tagged gene expression in stable cell line?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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Are these cassettes suitable for expressing PETase mutant in E. coli?

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EDC carbodiimide and NHS easter 's half life among different pH?

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Ajoy Paul

Mr. P.N.

Follow the paper on the link. this could help you.

http://journal.frontiersin.org/article/10.3389/fmicb.2014.00172/full

John Schloendorn

No. It's no use. Most likely the T7 promoter is just left there as an artifact from cloning, or perhaps because it makes a well-behaved sequencing primer.

Perumal Nandhakumar

Thank you to all for the kind Reply... i was about to waste my time with prokaryotic expression with that vector....