Perumal Nandhakumar

3 Questions 7 Answers 0 Followers

Questions related from Perumal Nandhakumar

How to amplify less copy number gene in Realtime PCR?

I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...

26 November 2017 8,250 2 View

Why the RNA got degraded during purification ?

I am trying to isolate a mRNA (1400 bases) from Dog placenta with trizol reagent. Every time we are getting degraded RNA. We have stored the 100mg sample in 300ul trizol reagent and stored at -80....

30 March 2017 1,241 2 View

Is it possible to amplify a single copy of dna template (practically) efficiently by using PCR ?

Theoretically, PCR can amplify a single copy of DNA into millions copy. Is it possible ?

15 March 2017 4,118 11 View