7 Questions 13 Answers 0 Followers
Questions related from Perumal Nandhakumar
I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...
26 November 2017 8,331 2 View
After second passage, vero cells stops growing and was showing granulation. I am using 10% FBS in DMEM along with antibiotic. Flask was filter capped and it was kept in 37 incubator with 5% CO2....
23 April 2017 4,332 3 View
One of the eukaryotic expression vector having CMV promoter followed by T7 promoter and kozak sequence. Can i express this protein in BL21 DE3 cells ? Note: There is no Shine-Dalgarno sequence...
20 April 2017 8,369 3 View
I am trying to isolate a mRNA (1400 bases) from Dog placenta with trizol reagent. Every time we are getting degraded RNA. We have stored the 100mg sample in 300ul trizol reagent and stored at -80....
30 March 2017 1,311 2 View
whenever i am doing subculture in 25cm2 flask, most of the cells are concentrated either the neck region or at the center of the flask (80% confluent at center and 30% confluent at remaining area...
22 March 2017 5,468 2 View
I want to express some protein in eukaryotic expression system. my gene of intrest has been cloned into eukaryotic expression vector (CMV promoter). But i dont have positive control vector to...
17 March 2017 1,234 2 View
Theoretically, PCR can amplify a single copy of DNA into millions copy. Is it possible ?
15 March 2017 4,189 11 View
