3 Questions 7 Answers 0 Followers
Questions related from Perumal Nandhakumar
I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...
26 November 2017 8,246 2 View
I am trying to isolate a mRNA (1400 bases) from Dog placenta with trizol reagent. Every time we are getting degraded RNA. We have stored the 100mg sample in 300ul trizol reagent and stored at -80....
30 March 2017 1,239 2 View
Theoretically, PCR can amplify a single copy of DNA into millions copy. Is it possible ?
15 March 2017 4,117 11 View