I bought human CD14 + monocytes from mobilized blood (10 million cells) to generate M1 and M2 macrophages. After thawing the cells, I wonder if I can expand them (1 passage) to make stocks before starting induction. I plan to use RPMI 1640, 10% FBS, 1% P / S. Thank you!
Malcolm Nobre
Hello,
I suppose monocytes are sensitive to freezing. Although on thawing they may be viable, they may lose some of their functions.
I suggest you freeze human PBMCs, and when you require monocytes you can thaw PBMCs and isolate monocytes using CD14 magnetic beads.
Best.
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