ATCC recommended media for HUVEC culture is F-12K medium (ATCC 30-2004). Is this of same composition of DMEM? Will my cells be able to grow properly in DMEM (supplemented with FBS) ?
Hello Swarnadip Ghosh
Probably not.
Originally, HUVEC cells were cultured in M199 supplemented with 20% FBS and 2 mM glutamine. But with the passage of time the formulations have improved with the addition of growth factors and reduction of serum concentration.
You may refer to the paper attached below.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC302542/pdf/jcinvest00186-0087.pdf
I would recommend that you use DMEM: F12 media. DMEM alone is rich in nutrients such as amino acids and vitamins, while Ham’s F-12 contains a higher diversity of components. Therefore, DMEM F12 is a more complex media than DMEM, including many additional factors (e.g., zinc, thymidine) that are generally not found in more standard basal media formulations.
DMEM: F12 (DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12) can support higher growth rates. This growth medium will combine the higher concentrations of components in DMEM with the wider range of Ham’s F12 ingredients.
You may prepare your own endothelial growth medium for HUVEC. The recipe is as follows:
DMEM/F12- 50:50
2% heat inactivated FBS
1 µg/ml hydrocortisone
5 ng/ml EGF
10 ng/ml FGF-2
20 µg/ml heparin sulphate
250 ng/ml insulin
100 U/ml penicillin
100 µg/ml streptomycin
Best.
Hi ....
Human Umbilical Vein Endothelial Cells (HUVECs) can be cultured in a variety of cell culture media. The recommended media for HUVEC culture is F-12K medium (ATCC 30-2004), which is specifically formulated for the growth and maintenance of HUVECs. However, it is also possible to culture HUVECs in other media, such as Dulbecco's Modified Eagle's Medium (DMEM).
The composition of DMEM is different from that of F-12K medium, and it may not provide all of the necessary nutrients and growth factors for optimal growth of HUVECs. Additionally, DMEM may contain higher levels of glucose and other components that could affect the growth and proliferation of HUVECs.
If you decide to culture HUVECs in DMEM, you will need to supplement the medium with fetal bovine serum (FBS) to provide additional nutrients and growth factors. It is also important to follow good cell culture practices, including using sterile techniques and maintaining proper pH and osmolality.
It is generally recommended to use the recommended media for HUVEC culture whenever possible, as this has been optimized for the growth and maintenance of these cells. However, if you do decide to use a different media, it is important to carefully evaluate the potential effects on cell growth and proliferation.
I hope this information is helpful!
Hi Malcolm Nobre, thanks a lot for the detailed information.
A small clarification. If I use DMEM/F12 1:1 with added L-glutamate, do I need to supplement with FBS ? If so, then at what proportion ?
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