I have been working with HEK 293T cells so far for expressing a protein. I was however recently asked to try CHO cells since these may a better expression system for my protein which failed to be expressed in HEK cells.
I wish to find out if I can used the same culture medium that I used for HEK cells to culture CHO cells?
Thanks you.
Make sure the medium you use contains proline, as CHO cells cannot synthesize it. That may be an issue with DMEM/FBS. Most manufacturers make a specific 'CHO' medium for this reason.
http://en.wikipedia.org/wiki/Chinese_hamster_ovary_cell
Hello
CHO cells should be cultured in Ham’s F12K (ATCC suggestion) or DMEM modified with 10% FBS. If cells are not doubling every 14-17 hours, supplement the medium with 1-2% FCS.
Subculture Protocol for CHO:
CHO cells grow quickly and easily and cell count should have a doubled within 14-17 hours.
Rinse cells with 0.25% Trypsin/0.53mM EDTA
Add 3 mL of Trypsin-EDTA to flask and watch for cell layer dispersal under an inverted microscope. this should occur within 5-15 minutes. Do not agitate cells during this type. Agitation encourages clustering. If cells are not detaching, place in incubator for 5 minutes to facilitate dispersal.
Centrifuge cells and remove supernatant to remove Trypsin-EDTA
Add 6-8 mL of growth medium and aspirate cells with pipette, gently.
Add aliquots of cells suspension to culture vessels.
incubate at 37°C in 5% CO2.
Split confluent culture 1:4 to 1:8 every 4-7 days with trypsin/EDTA. Make sure to renew medium 1-2 times between sub culturing.
please , read the DOC and visit link may help you
Good Luck
http://www.cho-cell-transfection.com/cell-culture-information-and-protocol/
http://www.biotechniques.com/protocols/Cell_Culture/Growing-CHO-cells-in-a-benchtop-stirred-tank-bioreactor-using-single-use----vessels/biotechniques-185490.html
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