Can I complement data I used in a previous publication?

More Alexander Bontempo's questions See All

Why in BLI some dissociation curves increase in high affinity ligand?

Hi. I'm analyzing the kinetics of antibody antigen interaction. I have some that show very low Koff. The problem is in the dissociation phase the curve is not only stationary but increase....the...

11 December 2019 4,945 3 View

Why when i do a BN-PAGE of a single SEC peak fraction i get many bands?

hi, i'm doing a protein purification and i'm wondering what are the factors make a single SEC peak give many bands in a BN-PAGE.

08 September 2019 7,503 8 View

Is a expression plasmid for HIV env protein a BSL2 agent?

Hi guys. I'm facing a institutional divergence about the BSL of a expression plasmid carrying the HIV env gp160 protein. They are make trouble believing it is a bsl 2 agent. Is that true? If is...

06 July 2019 8,134 4 View

How can i estimate different peaks in a overlaping muly-peak graph?

Hi, i made a sec an the chromatogram is composed of overlaped distributions(peaks). How can i estimate the apportation of each singular poblation? Wich is the best software that also permit a...

10 November 2018 8,915 0 View

Why my mini prep is working but no my maxi considering same bacterial culture?

Hi i have a quiz for you guys. i don't know how to solve this trick. attached you ll find flow chart with my issue in dettails. Briefly, i tried to purify a plasmid trough maxiprep (two different...

10 November 2018 6,866 5 View

Nobody has a detailed protocol for CHO transfection with PEI?

Hi, this is my first time i try protein expression so i need a really complete protocol to perform CHO-K1 transfection using PEI scalable up to one liter culture. I need a protein expression for...

04 May 2018 2,314 3 View

Why hiv gp140 trimers in chromatography shown a peak between ferritin and thyroglobulin references?

I saw some gromatography graphs and i can't understand why gp140 trimers who should weight 412kDa shows the peak between ferritin (440kDa) and thyroglobulin (669kDa) references. Thank you.

31 December 2017 1,091 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View