Alexander Bontempo

8 Questions 25 Answers 0 Followers

Questions related from Alexander Bontempo

Can I complement data I used in a previous publication?

I compared 4 protein trough a methodology. In a previous publication I just showed the data for 3 of them. Now I want to publish the complete set as my interest is to present the 4th protein that...

01 January 2020 7,337 2 View

Why in BLI some dissociation curves increase in high affinity ligand?

Hi. I'm analyzing the kinetics of antibody antigen interaction. I have some that show very low Koff. The problem is in the dissociation phase the curve is not only stationary but increase....the...

12 December 2019 5,022 3 View

Why when i do a BN-PAGE of a single SEC peak fraction i get many bands?

hi, i'm doing a protein purification and i'm wondering what are the factors make a single SEC peak give many bands in a BN-PAGE.

09 September 2019 7,579 8 View

Is a expression plasmid for HIV env protein a BSL2 agent?

Hi guys. I'm facing a institutional divergence about the BSL of a expression plasmid carrying the HIV env gp160 protein. They are make trouble believing it is a bsl 2 agent. Is that true? If is...

07 July 2019 8,199 4 View

How can i estimate different peaks in a overlaping muly-peak graph?

Hi, i made a sec an the chromatogram is composed of overlaped distributions(peaks). How can i estimate the apportation of each singular poblation? Wich is the best software that also permit a...

11 November 2018 8,976 0 View

Why my mini prep is working but no my maxi considering same bacterial culture?

Hi i have a quiz for you guys. i don't know how to solve this trick. attached you ll find flow chart with my issue in dettails. Briefly, i tried to purify a plasmid trough maxiprep (two different...

11 November 2018 6,926 5 View

Nobody has a detailed protocol for CHO transfection with PEI?

Hi, this is my first time i try protein expression so i need a really complete protocol to perform CHO-K1 transfection using PEI scalable up to one liter culture. I need a protein expression for...

05 May 2018 2,379 3 View

Why hiv gp140 trimers in chromatography shown a peak between ferritin and thyroglobulin references?

I saw some gromatography graphs and i can't understand why gp140 trimers who should weight 412kDa shows the peak between ferritin (440kDa) and thyroglobulin (669kDa) references. Thank you.

01 January 2018 1,151 2 View