Hi, i made a sec an the chromatogram is composed of overlaped distributions(peaks). How can i estimate the apportation of each singular poblation? Wich is the best software that also permit a graphicarepresentatio?
I compared 4 protein trough a methodology. In a previous publication I just showed the data for 3 of them. Now I want to publish the complete set as my interest is to present the 4th protein that...
31 December 2019 7,393 2 View
Hi. I'm analyzing the kinetics of antibody antigen interaction. I have some that show very low Koff. The problem is in the dissociation phase the curve is not only stationary but increase....the...
11 December 2019 5,077 3 View
hi, i'm doing a protein purification and i'm wondering what are the factors make a single SEC peak give many bands in a BN-PAGE.
08 September 2019 7,633 8 View
Hi guys. I'm facing a institutional divergence about the BSL of a expression plasmid carrying the HIV env gp160 protein. They are make trouble believing it is a bsl 2 agent. Is that true? If is...
06 July 2019 8,245 4 View
Hi i have a quiz for you guys. i don't know how to solve this trick. attached you ll find flow chart with my issue in dettails. Briefly, i tried to purify a plasmid trough maxiprep (two different...
10 November 2018 6,977 5 View
Hi, this is my first time i try protein expression so i need a really complete protocol to perform CHO-K1 transfection using PEI scalable up to one liter culture. I need a protein expression for...
04 May 2018 2,436 3 View
I saw some gromatography graphs and i can't understand why gp140 trimers who should weight 412kDa shows the peak between ferritin (440kDa) and thyroglobulin (669kDa) references. Thank you.
31 December 2017 1,206 2 View
I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...
03 August 2024 6,070 3 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...
31 July 2024 9,697 0 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.
29 July 2024 4,971 1 View
The first step is to analyze a 2D molecular graph and implement partitioning techniques to calculate the topological indices. Secondly impose statically tools to generate QSPR model, may or may...
24 July 2024 9,644 1 View
The Louvain method – named after the University of Louvain where Blondel et al. developed the algorithm – finds communities by optimizing modularity locally for every node’s neighborhood, then...
23 July 2024 6,659 0 View
Let n∈ℕ, where the function f:A⊆ℝ𝑛→ℝ, 𝐴 is Borel, and (A,d) is a completely seperable space. What is the name of the functions where the graph of f in any (n+1)-dimensional box of A×ℝ is...
17 July 2024 258 1 View
I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...
16 July 2024 6,918 7 View