Why hiv gp140 trimers in chromatography shown a peak between ferritin and thyroglobulin references?

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Can I complement data I used in a previous publication?

I compared 4 protein trough a methodology. In a previous publication I just showed the data for 3 of them. Now I want to publish the complete set as my interest is to present the 4th protein that...

31 December 2019 7,246 2 View

Why in BLI some dissociation curves increase in high affinity ligand?

Hi. I'm analyzing the kinetics of antibody antigen interaction. I have some that show very low Koff. The problem is in the dissociation phase the curve is not only stationary but increase....the...

11 December 2019 4,945 3 View

Why when i do a BN-PAGE of a single SEC peak fraction i get many bands?

hi, i'm doing a protein purification and i'm wondering what are the factors make a single SEC peak give many bands in a BN-PAGE.

08 September 2019 7,503 8 View

Is a expression plasmid for HIV env protein a BSL2 agent?

Hi guys. I'm facing a institutional divergence about the BSL of a expression plasmid carrying the HIV env gp160 protein. They are make trouble believing it is a bsl 2 agent. Is that true? If is...

06 July 2019 8,134 4 View

How can i estimate different peaks in a overlaping muly-peak graph?

Hi, i made a sec an the chromatogram is composed of overlaped distributions(peaks). How can i estimate the apportation of each singular poblation? Wich is the best software that also permit a...

10 November 2018 8,915 0 View

Why my mini prep is working but no my maxi considering same bacterial culture?

Hi i have a quiz for you guys. i don't know how to solve this trick. attached you ll find flow chart with my issue in dettails. Briefly, i tried to purify a plasmid trough maxiprep (two different...

10 November 2018 6,866 5 View

Nobody has a detailed protocol for CHO transfection with PEI?

Hi, this is my first time i try protein expression so i need a really complete protocol to perform CHO-K1 transfection using PEI scalable up to one liter culture. I need a protein expression for...

04 May 2018 2,314 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View