As we know that we try to isolates endophytic fungi from plant that have medicinal use.But,after isolates the fungus,we try to ferment and get the compound from it and found out that there are no activity or less activity against the bad bugs.Inside a plant,there's a lot of microorganisms live together/symbiosis which make the plant very useful for human.So,mean that the microorganisms need each other to produce secondary metabolites to fight the bad bugs.
Dear,
The active compound to against the ESKAPE maybe the new compound from fungal metabolites + phytochemical agents...not only fungal metabolites, therefore you don't see any effect against ESKAPE from harvested broth media....you may wanna know what is the real active compound then look at the metabolic pathway of that fungi and see what you can do to make this agent active..eg. add other compound to block or activate that by product..
Not sure if this answer your question ^^
Yes, thanks Kraiwuth Kallawicha for your answer. You have giving me some idea so that I can plan my experiment on fungi I have here.
You and the read the following article "Enhanced camptothecin production by ethanol addition in the suspension culture of the endophyte, Fusarium solani, Bioresource TechnologyVolume 188, July 2015, Pages 251–257". In pure culture, most of the fungal secondary metabolite production is stopped or producing low quantity. So you have to add elicitors to induced the genes responsible for secondary metabolite production.
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