Heba Kamal Nabih Hanafi

7 Questions 8 Answers 0 Followers

Questions related from Heba Kamal Nabih Hanafi

How can I calculate Bax/Bcl2 ration using gene expression?

Dear. Experts I have the mean normalized copy gene number for both Bax, and Bcl2 genes. How can I calculate Bax/Bcl2 ratio for each treatment?, and is there an indication range for the calculated...

16 June 2021 6,639 2 View

Why did I obtain two different near melting temperatures in the same RT-PCR reaction?

I obtained 2 melting temperatures (75, and 82 C) for the same sample with specific confirmed primers (forward, and reverse) by RT-PCR. But, I have single Ct for each. Do you think there is a...

23 May 2021 7,229 1 View

How can I stain the isolated exosomes with PKH67 kit?

I have 100ul of exosomes in XE buffer of isolating kit. I want a protocol to label this 100ul of exosomes with PKH67 fluorescent cell linker kit. Thanks!

24 March 2021 5,590 4 View

What about the composition of serum free medium for cell culture?

I want to culture human HepG2 cells in serum free medium. Should I add all components of complete medium except FBS only?, and how long the cells will be alive in such free medium?

25 February 2020 3,425 2 View

What about the availability of human brain tumor cell lines in Egypt?

Please, I ask about the availability of human normal brain and glioblastoma multiforme cell lines in Egypt, could any one recommend me where can I find them?

30 May 2019 6,240 0 View

SW480 or Human melanoma cell lines in Egypt

Could any scientific researcher guide me to obtain SW480 or Human melanoma cell lines in Egypt. Thanks in advance

01 January 1970 5,817 2 View

What is meant by cumulative apoptosis?

Cells in sub-G1 phase of cell cycle are described as cumulative apoptosis. Could anyone illustrare more details about this type of apoptosis? Thanks!

01 January 1970 4,331 2 View