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Questions related from Dylan Lawrie
Our lab has some leftover TRI solution and column-based kit for RNA isolation. Is it possible to use them together? Could i use the TRI solution to homogenize the sample then followed by column...
29 November 2021 9,321 4 View
Is there any reason for i failed getting any bands on electrophoresis after i did plasmid isolation from E. coli DH5a? My plasmid is ~17 kb in size and i already checked the transformant with...
17 October 2020 7,034 5 View
When i do RNA isolation many would suggest that RNA isolation have to be done using refrigerated centrifuge. Is it really necessary? What if i use a regular centrifuge and works at room temp....
25 June 2019 2,162 7 View
I am trying to do my first real time pcr analysis for flowering gene expression. Does primers used in routine pcr different than primers used in real time pcr? What are the criteria for designing...
03 August 2018 1,895 3 View
