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Questions related from Dylan Lawrie
Our lab has some leftover TRI solution and column-based kit for RNA isolation. Is it possible to use them together? Could i use the TRI solution to homogenize the sample then followed by column...
29 November 2021 9,399 4 View
Is there any reason for i failed getting any bands on electrophoresis after i did plasmid isolation from E. coli DH5a? My plasmid is ~17 kb in size and i already checked the transformant with...
17 October 2020 7,131 5 View
When i do RNA isolation many would suggest that RNA isolation have to be done using refrigerated centrifuge. Is it really necessary? What if i use a regular centrifuge and works at room temp....
25 June 2019 2,222 7 View
I am trying to do my first real time pcr analysis for flowering gene expression. Does primers used in routine pcr different than primers used in real time pcr? What are the criteria for designing...
03 August 2018 1,967 3 View
Our lab have that old thermal cycler as a gift from another institution and i want to try doing pcr using that thermal cycler. Unfortunately I don't have a manual for that thermal cycler and I...
19 January 2017 3,673 5 View
I know the old fashioned method like isolating and staining, but I want to know if there's any easier and reliable method that I can use.
28 March 2016 8,875 4 View
