14 Questions 17 Answers 0 Followers
Questions related from Vicken Aknadibossian
Can i run a precast TBE native PAGE gel (made with tris pH 8.3) with 1x TBE running buffer of lower pH such as 7.0? Would the lower pH of the running buffer lower the gel pH if i pre-run the gel...
12 November 2020 3,405 4 View
I am trying to do an EMSA with unlabeled RNA an some proteins. RNA is about 700nt long in-vitro transcribed and the 2 protein are about 30 and 60 kDa with PIs 6-6.5. One of the proteins is the...
04 September 2020 6,189 6 View
I'm a molecular biologist, and i have a few projects coming up in transcriptomes and small RNA analysis. Can i get by without knowing any programming using user-friendly software such an Geneious...
11 May 2020 8,003 1 View
I want to go for whole genome sequencing using next gen Illumina but i have some problems. Firstly, the organism is a mollicute with a small genome and is an obligate plant parasite with low...
18 March 2019 8,298 4 View
If i am preserving cut tree shoot samples in distilled water in the fridge with no antibacterial compound, will bacteria grow just on the base of the cut, or will it spread up the shoot in the...
22 July 2018 3,372 5 View
Hello, I am designing to produce antibodies against 15aa peptides, these antibodies will be used for detection of a pathogen in plant. The problem is when i blast these 15aa sequences there are...
13 February 2018 6,762 3 View
Hi, I'm planning to express a membrane protein in a cell-free system, the Purefrex 2.0 one to be exact. I've tried absolutely everything and failed to do so in E.coli. They've put in their...
23 January 2018 1,218 6 View
Hey, So i have a 39kDa Membrane Protein, cloned into pRSET C and transformed into C43, sequenced to makes sure everything is there and made a glycerol stock of. From that glycerol stock i start a...
16 December 2017 7,141 3 View
If i Lyse cell overexpressing a membrane protein with sonication in 20mM NaH2PO4 and 500mM Nacl, firstly can i load it as it is in sds page or do i have to add SDS loading buffer? Either way ,...
17 November 2017 10,082 8 View
This resource states they use an ultrasonic bath for cell lysis : http://www.soltec.it/d1/en/ultrasonic-sonication-for-protein-extraction We have a different ultrasonic bath with a max frequency...
10 November 2017 4,286 3 View
After induction i am performing cell lysis by vortexing in 8M Urea to check for expression, and then centrifuging at 15000g for 10min, and my target protein is not in the supernatant, is it...
08 November 2017 3,180 3 View
So, i am double digesting pRSET A vector and an insert with BamHI and PstI ... running a gel and purifying the respective bands.... After ligation and transformation , and running PCR on the...
06 October 2017 3,787 3 View
I have just purchased a 12 rxn C43 competent cells kit. I want to make a stock from it and store in -80 . After i regenerate the cells by plating them out and culturing in liquid medium, to...
18 June 2017 901 2 View
If i were to use pQE30 vector which has a T5 promoter in a C43 competent cell which say "These cells can be used to express any gene cloned into a plasmid containing the T7 promoter". Will I get a...
10 April 2017 10,040 4 View
