I tried running cell lysate from epidermal keratinocytes on a gel during sds-page but realized that it is severely contaminated with genomic DNA - I am fairly certain this is because I did not use enough RIPA buffer during extraction which resulted in incomplete cell lysis. Is it possible to thaw these cell lysate samples and add more RIPA buffer followed by vortexing and centrifuging to get rid of nucleic acid contamination?
Total protein isolation using RIPA buffer has been an issue and may cause altered protein profile. Therefore, a strong buffer could be used to overcome this problem. Further, addition of more RIPA buffer again may cause dilution of your sample. Therefore, a quick sonication (just for 10 sec or so) is not a bad idea and may solve your problem at this stage. The Cell Signaling sites (as given below) also recommend the sonication. For more detail see the link given below:
http://www.cellsignal.com/common/content/content.jsp?id=western
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