Can HT22 cells be differentiated into Dopaminergic lineage?

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Problem with beta galactosidase staining assay: X-gal crystals in staining solution?

Hello, I'm conducting a beta galactosidase staining assay for my fibroblasts using X-Gal. I dissolve X-Gal (stock 50mg/ml prepared in DMSO) in the staining buffer at a final concentration of...

25 March 2024 8,799 3 View

If OIBs are uncommon during Archean due to higher melting degrees, why Carbonatites are found during Archean?

The absence of Ocean Island Basalts (OIBs) in the Archean rock records is commonly attributed to the higher melting degrees associated with mantle plumes. However, this explanation raises...

14 March 2024 2,195 1 View

What is the best method for endophytic bacterial isolation?

Mostly bacteria living inside plant roots.

14 March 2024 4,602 0 View

Can anyone share literature of AVO envelope attribute?

Can anyone share literature of AVO envelope attribute? You can reach me at [email protected] Regards Sabyasachi

29 November 2023 8,956 0 View

Counterpoise (BSSE) Correction for more than 2 fragments?

What is the correct way to calculate the BSSE correction for a molecule having 3 fragments? Please see the attached image for details. I know the values are pretty close but still I would love to...

25 October 2023 3,990 1 View

Can I use TRIX index for coastal lagoon using DIN and PO4 instead of TN and TP?

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26 September 2023 432 1 View

What was the mode of weathering/erosion during Hadean to Mesoarchean?

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19 September 2023 6,875 0 View

Has anyone studied locus specific 5-hydroxymethylation using Epimark 5mC and 5hmC kit?

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How to calculate the number carbon quantum dots (CQDs) internalized per cell using "IntraCell V2" plugin in ImageJ?

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01 September 2023 419 0 View

Why my pisoFoam solver is showing "Floating Point Error" can anyone help me in this regard?

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18 August 2023 2,311 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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09 August 2024 9,621 0 View

Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

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04 August 2024 4,090 0 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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Shibashish Giri

yes, it is possible. Expose them by lithium Chloride . With best wishes

Jordi Galiano-Landeira

Hi,

As a non-differentiated cells, HT22 line could be reprogrammed using a dopaminergic differentiated protocol as it is showed in the follow paper:

Kriks, S., Shim, J.-W., Piao, J., Ganat, Y. M., Wakeman, D. R., Xie, Z., … Studer, L. (2011). Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease. Nature, 480(7378), 547–551.