I have synthesized a drug molecule and now I need to purify and isolate it. Its difficult for me to do column chromatography. So I wanted to know if doing HPLC will serve the purpose or not. Thank in advance.
Bably
Read about semi-prep HPLC columns. Every Vendor offers such columns and provide its characteristics - including amount of injectwd sample.
Rwgards
Gb
yes, you can use for small quantities as HPLC is not designed to purify but to separate stuff. Also, HPLC is too expensive and better to look for alternative if you have a significant amount to be purified.
Thanks Udaya Jayasundara Jasim Al-saadi Grzegorz Boczkaj . I havenot done it yet. So can you please explain the maximum amount that can be used. Also about preparatory HPLC?
I have tried to modify the structure of a drug molecule by refluxing method. I think if I get a single product spot in TLC I should not go for purification. Please correct me if I am wrong.
Bably
Read about semi-prep HPLC columns. Every Vendor offers such columns and provide its characteristics - including amount of injectwd sample.
Rwgards
Gb
As everyone suggested, yes you can on a relatively small scale. TLC will guide you on solvent selection. Consider the column carefully abd choose a scale and condition based on the compound and column.
Why you didn't consider using solvent recrystallization to purify your product before resorting to column chromatography or to the more expensive preparative HPLC?
Yes. See attached:
https://www.thermofisher.com/ca/en/home/industrial/chromatography/liquid-chromatography-lc/hplc-uhplc-columns/preparative-hplc-columns.html?ce=E.19CMD.CC120.22422.01&cid=E.19CMD.CC120.22422.01&en=CMD_XX_PrepLCColumn-APR-3rd_E65691_PD_1906_BD_SC_CC120
Don't forget you need a fraction collector at the end of the purification chromatography in order to get the 'pure' molecule in the mobile phase.
So, in addition to Bruce - remember about transport delay (you need to determine it for you system) between the detector and fraction collector. If you need one fraction - you can collect it manually using the detectors outlet capillary.
If you install the fraction collector make sure that no extra big overpressure will be present in the detector - the detector cell has a pressure limit.
Regards
GB
Pleses see below link:
https://www.thermofisher.com/ca/en/home/industrial/chromatography/liquid-chromatography-lc/hplc-uhplc-columns/preparative-hplc-columns.html?ce=E.19CMD.CC120.22422.01&cid=E.19CMD.CC120.22422.01&en=CMD_XX_PrepLCColumn-APR-3rd_E65691_PD_1906_BD_SC_CC12
Qualitatively you can but not quantitative. HPLC is not an efficient tool for separation, due to its restriction for column loadability, use of buffers and additives as mobile phase it becomes more difficult for separation and isolation. Alternately, you can use TLC having stationary phase C18 for isolation on a small scale or else you can go for preparative HPLC.
Preparative HPLC have been used for years for purification purposes. There is several vendors who sell semi and preparative columns for this purpose. Just try to select a mobile phase with not too many additives so it will be easy to evaporate the mobile phase and get the pure product. As it was said to have to determine the time when you collect the fraction containing the compound of interest been with a fraction collector or manually at the end of the detector output. You can test the purity of the fraction collected by TLC or by HPLC
You can use Prep-HPLC for this purpose and it will be much better than CC.
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