If yes how can we compare the effectivity of a newly synthesized sample with the previous ones?
while doing MTT assay in human PBMCs with doses increasing from 25microgm/mL to 100 micro gm/mL, I have found an increasing trend in cell viability. I could not find any reference or the reason...
09 October 2019 1,731 2 View
I have attached the method as well as calculations for drug release but could not find appropriate results even after several repetitions. It was clearly visible that there must be a high...
09 October 2019 8,339 3 View
MTT assay demonstrated that there is no effect of a curcumin derivative at 24 h but shows good effectivity at 48 h. I think this might be due to the slow dispersion of the drug in the cell culture...
09 October 2019 9,841 2 View
I have studied the swelling behaviour and drug release pattern from hydroxypropyl beta cyclodextrin where I have found that the swelling and release % was more in basic compared to acidic. It...
06 July 2019 7,856 2 View
I have synthesized a drug molecule and now I need to purify and isolate it. Its difficult for me to do column chromatography. So I wanted to know if doing HPLC will serve the purpose or not. Thank...
05 June 2019 6,547 18 View
I have tried to change the structure from CUR to CP. I know XRD is not mandatory here but since I did I would like to analyze the result. A very simple explanation will also be highly appreciated....
03 April 2019 2,526 8 View
While preparing a drug loaded polyelectrolyte complex nano/micro particles using two oppositely charged polymers, the particle starts forming after the addition of second polymer (precipitation...
01 January 1970 9,258 10 View
The manuscripts in my field (polymeric Drug delivery) includes mostly images, a few tables and 2-4 equations. Also formatting the manuscript into different journal type and managing references is...
01 January 1970 7,935 7 View
Can you please recommend me a polymeric nanoparticle system to load a partially hydrophilic drug for controlled release or tune the release behaviour
01 January 1970 2,830 4 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...
27 February 2021 8,483 3 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...
26 February 2021 6,905 10 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...
24 February 2021 7,470 7 View