Can HIV drugs will be a provisional or recurring option against COVID-19?

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How can I make complex file of ligand (including flexible residues from protein) with protein Structure after AutodockFR docking?

I have performed the Molecular Docking using ADFR suite, I have obtained the files in _out.pdbqt format for ligand docked poses, upon inspection, both visually and reading the pdbqt files of...

08 June 2024 8,130 2 View

How does uplink IM-NOMA works?

I am currently studying index modulation aided NOMA technique but my mind blown up with mathematical expressions. Can someone explain to me these; How Index Modulation aided NOMA (IM-NOMA) works?...

27 April 2024 2,736 1 View

Does non terminal his-tag work in purification?

i am working with membrane protein and there is a his tag 4 amino acid above the c terminus (seq HHHHHHDLEY). anti his antibody does not recognise the his tag in western blotting. i was wondering...

14 April 2024 2,675 5 View

How to interpret liquid chromatography mass spectrometry (LC-MS) result?

I have done the LC-MS result of my complex and its molecular weight is supposed to be 515.5 g/mole. I want someone who is experienced in LC-MS data interpretation. I have attached the picture of...

16 February 2024 382 2 View

How to slow down 1165 based lift-off for thermally deposited metals on semiconductors ?

quick summary: I am looking for a way to slow down lift-off (1165 or acetone) for a given material system (changing materials to solve the problem is not possible) This is a relatively general...

08 January 2024 8,955 2 View

How to Report an Authorship Misrepresentation?

Dear ResearchGate Support Team, I hope this message finds you well. I am writing to bring to your attention a concerning matter regarding the authorship of certain articles on ResearchGate. It has...

25 November 2023 3,908 1 View

while getting data that whether newts can be sustainbale in farm land ponds in compare with natural reserve?

how can we calculate average heterozygocities and unique alleles also how we can compare both populations of different ponds?

21 November 2023 3,238 1 View

Any genetics expert? or the person who is doing research on newts?

i need assistance with few terms your help will be appreciated.

21 November 2023 4,398 1 View

What are the hot topics for Research in Machine Learning in the field of Computational Science and Engineering?

Using machine learning algorithms

26 July 2023 3,025 2 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

Why not we are following sars COVID 19 guidance?

If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.

19 July 2024 3,356 2 View

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View