Does anyone has experience in quantifying tube length and branching points in H&E images of matrigel plugs using angioanalyzer of imageJ or any other freely available software?
Dear Mallu Abhiram Charan Tej - H&E staining can be used to quantify angiogenesis. You would need to ensure that the section size is identical between your experimental and control samples (in case you are performing a comparison).
Just a suggestion, but Mallory Trichrome staining might give you better clarity visually as the erythrocytes are stained a distinctive red in a blue-ish background.