After ExonucleaseI digested a strand of 58bp and incubated at ssDNA,37 ℃ for 2 hours and inactivated 15min at 85 ℃, the negative value was still very high after fluorescence verification. It is necessary to know whether this exonuclease I can completely degrade this single strand into a single nucleotide, and whether it can be completely degraded. I do not know if you have encountered this exonuclease I degradation is not clean or single-strand degradation is not complete, ask God for advice.
Tahreer M. Al-Thuwaini
This 3' to 5' exonuclease is highly specific for single-stranded DNA and does not react with double-stranded DNA or RNA. Exonuclease I is inactivated by heat treatment at 80°C for 15 minutes.
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