Kang Zong

3 Questions 2 Answers 0 Followers

Questions related from Kang Zong

Gene editing knockin cells have fluorescence, but pcr has no target band?

May I ask why the cells have fluorescence after the target gene is knocked in by the gene editing method, and the genome is extracted after the monoclone is screened, and then PCR is performed,...

21 August 2023 5,932 3 View

What is the reason for adding an intron between the homologous arms of the donor vector designed by gene editing? Why put the puro gene in the intron?

What is the reason for adding an intron between homologous arms in the donor vector designed by crispr/cas9 gene editing knockout experiment? Why put the puro gene in the intron?

17 August 2023 4,502 2 View

Can donors be inserted into the genome in reverse? What does it do?

Can DNA donors repair template be inserted into the genome in reverse? What does it do?

16 August 2023 5,564 1 View