Well, the human mitochondrial polymerase γ, DNA polymerases ε and δ from S. cerevisiae, and E.coli DNA polymerase III simply read-through uracil whereas DNA pol from thermophilic archaea and mesophilic archaeon M. acetivoran cease DNA polymerization in response to template strand uracil.
In a Polymerase chain reaction it will identify the Uracil as a thymine and pair it with adenine.
Expanding a bit on Syed's answer: PolA-type polymerases such as Taq will happily work with deoxyuridine-containing templates. PolB-type enzymes such as Pfu, on the other hand, will not. That's why some dNTP stocks that work fine with Taq do not work with Vent or Pfu (due to contamination with dUTP arising from deamination of dCTP).
TurboPfu, if I recall, is simply a preparation of Pfu containing a thermostable dUTPase to bypass this problem.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques