I am trying to use DNA FISH to detect plasmid DNA after transfection. I am curious if one should expect to see signal in the cytoplasm only or cytoplasm and nuclei? Any literature suggestion is highly appreciated.
Hi, I think it depends on your sequence , therefore you should select a unique sequence only found in plasmid , maybe you can target origin of replication site of the plasmid.
Hello,
I would suggest you shoud follow this paper, hope you wil find your answer.
"Fluorescence in situ hybridization to monitor the intracellular location and accessibility of plasmid DNA delivered by cationic polymer-based gene carriers"
Article Fluorescence in situ hybridization to monitor the intracellu...
I would suggest you the below paper
https://www.pnas.org/content/88/18/7953.short
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