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Questions related from Sneha Paul
I am trying to use DNA FISH to detect plasmid DNA after transfection. I am curious if one should expect to see signal in the cytoplasm only or cytoplasm and nuclei? Any literature suggestion is...
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Generally, for G-Quadruplex labelled with FAM and TAMRA FRET pairs it is a convention to monitor the changes in FAM emission (530 nm) to construct the FRET melting curves. Is there any specific...
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I wish to carry out FRET melting as well as smFRET experiments on G-Quadruplex DNA. The temperature range for melting assay would be 15-95 oC. Most of the papers use cacodylate buffer but I am...
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