Sneha Paul

3 Questions 5 Answers 0 Followers

Questions related from Sneha Paul

Can DNA FISH be used to detect plasmid DNA?

I am trying to use DNA FISH to detect plasmid DNA after transfection. I am curious if one should expect to see signal in the cytoplasm only or cytoplasm and nuclei? Any literature suggestion is...

08 February 2022 3,070 3 View

Can TAMRA (acceptor) emission be used to determine Tm for G-Quadruplex FRET melting assay?

Generally, for G-Quadruplex labelled with FAM and TAMRA FRET pairs it is a convention to monitor the changes in FAM emission (530 nm) to construct the FRET melting curves. Is there any specific...

31 May 2018 4,860 3 View

Is phosphate buffer a correct choice for performing FRET melting assay for G-Quadruplex?

I wish to carry out FRET melting as well as smFRET experiments on G-Quadruplex DNA. The temperature range for melting assay would be 15-95 oC. Most of the papers use cacodylate buffer but I am...

28 May 2018 3,592 4 View