Can CYC1 promoter of Saccharomyces cerevisiae be functional in Pichia pastoris?

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For Amyloid beta ELISA should i use the supernatant or the cells?

i am planning to do ELISA for AB quantification in an Alzheimer related project. the sample is cell culture (not animal samples), since AB deposits in the extracellular environment should i use...

14 May 2024 5,244 3 View

What is the proper solvent for super absorbent polymer (sap)?

I need to dissolve sap, what is the proper solvent?

20 August 2023 7,831 1 View

Whats the proper medium for dispersing chitosan nanoparticles during zeta potential measurement?

I prepared chitosan nanoparticles and while measuring zeta potential I tried to suspend them in distilled water but they precipitated immediately and I didnt have enough time for measuring zeta...

28 July 2023 9,636 3 View

I synthetized chitosan nanoparticles loaded with vitamin B12, PDI of drug-loaded nanoparticles was lower than pure ones, whats the explanation?

decrease of PDI occurred concurrent with mean particle size elevation.

24 March 2023 2,768 1 View

Is it possible to substitute core and shell in the synthesis of core-shelled Spinel ferrites?

I'm currently studying the synthesis of core-shell spinel ferrites. Most commonly, the core is spinel ferrite, and the shell is metal oxides (such as TiO2, SiO2, etc.). My question is, can the...

28 October 2022 7,464 2 View

Any good quality assessment tool for assessing quality of systematic reviews ?

Please suggest any good tool to evaluate the quality of systematic reviews for conducting systematic review of systematic reviews.Thank you in advance.

11 September 2022 166 4 View

Is GelMa FDA approved?

I think FDA wouldn't approve raw materials, so if that's the case, has GelMa been used in any FDA approved device/drug for human purposes? For having shorter path of approval for similar...

15 July 2022 2,655 0 View

How Watershed algorithm is made?

Hello dear researchers How to do the watershed algorithm in eCognition developer software? Do I need to add a plugin to watershed segmentation? How? There is no watershed segmentation plugin in my...

13 December 2021 7,129 2 View

Can arabinose inducible promoter be engineered in yeast?

does someone know a paper where authors engineered the arabinose induction system of prokaryotic organisms in yeast Saccharomyces cerevisiae, like an IPTG induction system, for orthogonal gene...

03 November 2021 7,860 3 View

How to measure the dryness of trees?

Hello In my work, I want to measure the state of tree drying using statistics. Other than measuring the percentage of dryness visually, can you introduce me to a method? If you can introduce me...

30 October 2021 9,954 8 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

30 July 2024 1,412 8 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View