23 June 2014 1 7K Report

Can sequence L/V-X(1-5)-Y-X(1-5)-R/K that is cholesterol binding motif be found in cytosolic proteins? Does it contribute to membrane targeting of these proteins? Can anyone suggest corresponding articles?

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How to convert IU/ml to ug/ml in case of anti-TSHR antibodies?

Does anybody know how to convert IU/ml in case of anti-TSHR antibodies (Graves disease)? I would like to know how much anti-TSHR Ab I have in a sample

02 February 2024 1,625 3 View

Dear All, do you have any idea regarding dating methods of beach rocks (I mean cement from a carbonate conglomerate, age: last 100 years)?

I expected age between present up to 100 years. I heard U-Th is useless in this case. If you have any idea let me know, please.

27 January 2024 8,273 7 View

How to choose antibodies for western blot?

What are the rules when choosing antibodies

04 September 2023 4,832 4 View

Where can I find examples of practical use of randomly distributed natural fibers to improving the soil in situ, no in the laboratory tests?

I am looking for examples of practical use of randomly distributed natural fibers to improving the soil, e.g. under the foundation or as the top layer of a dirt road, or other. In the literature,...

22 June 2023 340 0 View

Thermal degradation of polystyrene using QMS system: what does m/z=39 come from?

Hello everyone, I would like to ask about thermal decomposition of polystyrene using TG-QMS system - thermogravimetry coupled to quadrupole mass spectrometer. During thermal degradation of pure...

14 February 2023 8,556 3 View

What is the best anti-CD8 antibody for IF of paraffin sections of human cancer?

Dear Fellow Researchers, Can you recommend a suitable antibody recognizing human CD8 in paraffin sections of cancer samples? We want to combine it with other markers and do an analysis by...

09 October 2022 3,676 1 View

How to use continuous variables in COX regression?

Dear Fellow Researchers, We have analyzed the effect of various parameters on bladder cancer recurrence. Among included parameters, we had various continuous parameters. In the literature,...

20 August 2022 6,514 4 View

What are the suspended minerals paricles in seawater?

I am interested in the minerals particles suspended in seawater. What are the minerals? Carbonates, silicates, what more? Which clays are the most common? Could someone provide articles related to...

19 August 2021 6,542 6 View

Does anybody know how to coat Corning microcarriers with Matrigel?

I got CLS4621 and Corning Matrigel 356234

23 May 2021 974 0 View

Concerning Mossbauer spectroscopy: large QS for Fe3+ in Fe-silicates ? It is possible in minerals?

After fitting Mossbauer spectra for Fe(II)-silicate heated at air to 700degC, I observed uncommon, very high quadrupole splitting parameters for Fe3+, I suspect in very disordered environment (?)....

03 May 2021 5,143 5 View

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Is artifacts in XPS possible to build high deviation in binding energy larger than 5 eV??

Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...

30 July 2024 9,824 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

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What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

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Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

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