Can I use conventional PCR primers for qPCR for virus quantification? i don t have any experience for qPCR and I got good band by using those primers in conventional PCR , MCP gene was cloned into plasmids and plasmid DNAs was extracted.i wanna to know is it possible I use conventional PCR primers for qPCR ?I am waiting for your opinion and comments.
Hi, Maryam, theoretically yes, you can use. But it can dependent form your primers details: length of the fragment, temperature etc.. The main point is the fragment length; for qPCR fragment size must be quite short; 100.150 bp.
You can also look on previous discussion on the same topic: https://www.researchgate.net/post/Can_primers_for_RT-PCR_be_used_to_do_the_SYBR_fluorescence_quantitative_qPCR
Hi, Maryam, theoretically yes, you can use. But it can dependent form your primers details: length of the fragment, temperature etc.. The main point is the fragment length; for qPCR fragment size must be quite short; 100.150 bp.
You can also look on previous discussion on the same topic: https://www.researchgate.net/post/Can_primers_for_RT-PCR_be_used_to_do_the_SYBR_fluorescence_quantitative_qPCR
- Badges
- Science method