Would you please guide me bout capillary zone electrophoresis?
1- Can capillary zone electrophoresis method be useful to determine the concentration of Calcium, magnesium, sulfate and citrate in solution?
It is possible to use CE for this purpose but you need a suited detector. C4D will do the job. If you have a little bit of technical support it is possible to build it yourself. C4D stands for 'capacitive coupled contact less conductivity detection'
An exemple:
https://www.researchgate.net/publication/215580430_7-_Development_and_validation_of_a_chromatographic_and_electrophoretic_method_for_the_determination_of_amikacin_and_urea_in_bronchial_epithelial_lining_fluid?ev=prf_pub
Article Development and validation of a chromatographic and electrop...
If you have a CE system with an absorbance detector, you can use indirect absorbance with good results. Please see: https://www.beckmancoulter.com/wsrportal/bibliography?docname=IB-17136.pdf
For the purpose you need two methods: one for cations and another one for anions. If you are not familiar with CE, especially with method development, I suggest you to apply Analis CE kits for the separations.
http://www.analis.be/bin/site/render.cgi?id=0051161_item_to_sell&ln=ln1&userid=&rubr=ceofix&search=
CE is very good at ion separations. Normally cations and anions are separated in differenct conditions. EOF must be well controlled for anion separations, since normally nontreated fused silica capillary has EOF in opposite direction to anion electrophoresis migration. Detector is another factor to be considered, as aforementioned, either conductivity or indirect photometry methods.
CE can be used for separation of inorganic cations and anions. For cations usually MES/ His buffer ~6 pH is used, however, you need C4D (capacitively coupled contactless conductivity detector) detector. Capacitance-to-digital detector can also be used:
https://www.researchgate.net/publication/260411113_Capacitance-to-Digital_A_Single_Chip_Detector_for_Capillary_Electrophoresis
similar buffer can also be used for separation of inorganic anions, however, you need to add CTAB for reversal of electroosmotic flow.
If you need more technical details about building capacitance-to-digital detector leave a message. It is much simpler than C4D.
Article Capacitance-to-Digital: A Single Chip Detector for Capillary...
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH