knockdown technique
Hi, there are 2 bands in my protein's MW. What could be the reason for shifting the band from the original band? My protein's molecular weight is 131 KDa and it is bound with a FAD cofactor. I...
28 May 2024 4,294 1 View
I want to produce water stress in a potted plant. Whats the easy method?
13 May 2024 3,910 3 View
Hello I am currently engaged in research focusing on plant genetic transformation. As part of this endeavor, I have designed a comprehensive in silico plasmid cloning approach. The aim is to...
07 May 2024 5,119 3 View
Hello, I hope this message finds you well. I am currently planning to synthesize a long chain oligonucleotide using our oligonucleotide synthesizer. However, I find myself uncertain about the...
07 May 2024 7,460 1 View
n is time. and i want to know if the distribution of small scale fading is correct or not.
06 May 2024 4,971 11 View
n= time. find the distribution for small scale fading variable.
06 May 2024 352 0 View
I am working on the Primary neuron cell. I am trying to observe the stress granule in the cell. For that, I have done transfection with my gene of interest and treated the cell with Arsenite. I...
17 April 2024 790 1 View
I get \lambda_i in the numerator when I integrate instead of \lambda_1.
13 April 2024 5,199 3 View
13 April 2024 9,844 3 View
To quantify the stoichiometric homeostasis index for N (nitrogen). The formula is as follows: log (y)= log(c) +(1/HP)log(x) (4) where y is the N level in the plant tissue (mgkg−1), x is the N...
10 March 2024 1,605 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
or can you recommend articles showing the differential effect of LPS in a time dependant manner?
28 July 2024 2,547 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
