Can anyone tell me the steps for PMR calculation in detail? Or is there any other way to calculate pmr value in methylight reaction?

01 January 1970 0 4K Report

As i understood these are the steps for PMR calculation- Step 1. Divide mean values/ copy (gene) by mean values/ copy (alu) for the test sample Step 2. Then do the same for M.sss1 treated DNA Finally divide step 1 by step 2 and multiply with 100. The mean values / copy for alu will be Calculated via standard curve but how I can calculate the mean value/ copy for the test gene? Should I keep the standard curve for each gene using m.sss1 treated DNA?can i do relative quantification instead of standard curve method?

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