I am currently isolating liver cancer stem cells from HCC cell lines by using magnetic cell sorting system. But after sorting I want to keep them in their undifferentiated state for my experiments.
In this artikal there is a good discribtion of media used for culturing cancer stem cells:
The secretome of colon cancer stem cells contains drug-metabolizing enzymes
Benjamin L Emminka et al.
advanced Dulbecco's modified Eagle's medium/F12 (Invitrogen) supplemented with 0.6% glucose (BDH Lab. Supplies), 2 mm l-glutamine (Biowhittaker, Rockland, ME), 9.6 μg/ml putrescin (Sigma), 6.3 ng/ml progesterone (Sigma), 5.2 ng/ml sodium selenite (Sigma), 25 μg/ml insulin (Sigma), 100 μg/ml apotransferrin (Sigma), 5 mm HEPES (Invitrogen), 0.005 μg/ml trace element A (Cellgro), 0.01 μg/ml trace element B (Cellgro), 0.01 μg/ml trace element C (Cellgro), 100 μm β-mercaptoethanol (Merck), 10 ml antibiotic–antimycotic (Invitrogen), 4 μg/ml gentamicine (Invitrogen), 0.002% lipid mixture (Sigma), 5 μg/ml glutathione (Roche), and 4 μg/ml Heparin (Sigma). Growth factors (20 ng/ml EGF (Invitrogen) and 10 ng/ml b-FGF (Abcam, Cambridge, UK))
you ca also try to use kubota medium that is designed for normal liver stem cells.
It is produced by phoenix songs:
http://shop.phoenixsongsbio.com/Kubotas-StemCell-Growth-Media-250ml-11001-250.htm
Hello
All suggestions its good , and you can use the following medium:
# Human Liver Cancer Stem Cell Culture catalog
# Human Liver Cancer Stem Cell – Frozen Vial
in attachment description of these medium , I hope that is good for you
Best wishes
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