Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

How can i send the Mandelys reference Library file ???

hi to all my dear colleagues, i need your help concerning finding the proper way to send and prepare the library file of references by Mandely??

06 July 2020 383 5 View

I would like to quantify cytokines from mice brain using ELISA, What is the total protein concentration i suppose to add in each well?

Hi everyone I want ant to use ELISA to quantify tnf a ,il-1b and inf g in Half brain of mice. I was wondering if any one has done this exactly before and can tell me which concentration of...

07 May 2019 7,701 3 View

How to deal with absent of standardization in methodology to carry-out new study?

Hi, I will be using image processing techniques to predict treatment outcomes to some kind of tumors. I'm about to design my study, So, I went through many papers and literature that has...

25 March 2019 2,348 2 View

Does BHK (Baby Hamster kidney) cell line have specific regulations to Enter Egypt?

I need to import BHK cells to Egypt, but I'm not sure if there are any regulations for it to Enter!

26 February 2019 4,814 1 View

What does it mean by negative values for number of genotypes identified by a primer in detecting the polymorphism ?

So i am recording the polymorphic statistics of iPBS primers and one of the parameters estimated is R (resolving power). From an equation (0,15x + 1,78 = R) i can detect the number of genotypes...

14 February 2019 9,111 1 View

What is the different between UPGMA and Neighbor-Joining Methods in the building of the phylogenetic tree?

the methods of building the phylogenetic tree

12 April 2017 1,050 2 View

Can anyone provide me with some papers regarding the nutrient deficiency within the soil in Iran ?

29 January 2017 650 4 View

Vitamin B12 detection & determination?

What is the best method to detection and determine of Vit. B12 from bacteria?

10 November 2016 6,035 3 View

How can you verify if your research work is novel or not?

03 March 2016 9,154 33 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View