Can anyone tell me a protocol for mixture of DNA (90bp) separation of same size ?

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Shivakumar Rayavara

Dear Burzynski,

Thank you for the reply.

We are trying that, also we are looking for some technoques where we can separate by page (dye based). it would be gratea if you could give few suggetions (possible protocol) on these techniques.

Thank you Burzynski,

Let me try with TA cloning vector first.

Yogesh Chaudhari

Try Denaturing Gradient Gel electrophoresis (DGGE)...you will get 10 different bands, if products of approx. same size differed in base sequences..

Dear Chaudhari,

Thank you for the suggestions.

Elena A Usacheva

You can try SSCP approach. See also paper:

http://genome.cshlp.org/content/1/1/34.full.pdf