Hi,
I need to extract the DNA of copepod and I'm planning to use Phenol Chloroform method. Since, I feed the copepods every day, I need to prevent contamination of Algal DNA while performing the procedure. One of the researcher suggested not to feed copepods for two days before I perform the procedure. Does it work ?
Prompt reply is really appreciated
Thanks in Advance
HI, I would say it depends on what is your aim or next application?
Will you use copepod-specific primers on your DNA? Then you will not be in trouble with some algal DNA. Or you feed them and sample after ~12h or when ever the food is digested, depending on the species you can probably see the gut content and processing ;-) Not feeding copepods sounds like a bad option but also depends on what you want to investigate. Starving them will impair their constitution and probably various physiological functions (egg production, growth...) and cause a lot of stress of course!
I appreciate your prompt suggestions and thank you very much for information Janina Rahlff , Artur Burzynski, David Farringdon Spencer. I will try as per your suggestions and update it if there is any need or suggestion in near future.
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