Can anyone suggest the manual protocol for metagenome isolation from hot spring water ?

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Arvind Behal

Will you take water as sample or soil from sides along the flowing water?

water sample can be filtered on 0.22 micron filter, then use this filter to isolate DNA.

Nataly Guevara

Attached you will find a very well described protocol for metagenome isolation from sea water. If you need more specific information, I can send you a protocol that I used to extract DNA from hydrothermal vents.