Can anyone suggest the manual protocol for metagenome isolation from hot spring water ?

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Does anyone have an idea regarding the selection of hypervariable regions of 16S rRNA gene for pyrosequencing?

Microbial diversity study.

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How do I check the quality of my viral RNA prior to metagenomic sequencing?

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Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

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Resume Book UEP2013 Asian Edition, dis somebodey has it?

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Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

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Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

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Arvind Behal

Will you take water as sample or soil from sides along the flowing water?

water sample can be filtered on 0.22 micron filter, then use this filter to isolate DNA.

Nataly Guevara

Attached you will find a very well described protocol for metagenome isolation from sea water. If you need more specific information, I can send you a protocol that I used to extract DNA from hydrothermal vents.