Amitsinh Vijaysinh Mangrola

11 Questions 16 Answers 0 Followers

Questions related from Amitsinh Vijaysinh Mangrola

Can anyone help me on substrate selection for lipase assay?

I am working on lipase production. After literature search, i got information about different substrates used for lipase assay i.e., Peranitrophenyl palmitate, Peranitrophenyl laurate,...

27 August 2014 3,623 2 View

Does anyone have idea regarding silver nanoparticle synthesis from plant water hyacinth (Eichhornia crassipes)?

We are dealing with the work entitled Synthesis of silver nanoparticle from plant. I have faced problem with particle size analysis by DLS. We got silver nanoparticle size in the micron range not...

18 July 2014 925 5 View

Does anyone have an idea regarding isolation of microorganisms from hot spring environments?

We are dealing with culturable analysis of microbial community found in hot springs. Which procedures should I apply for studying cultivable microbial diversity and how can we start our work in...

15 July 2014 2,089 5 View

Where can I get workshop to learn about NGS- ION TORRENT and NGS Data analysis by Bioinformatics tools?

We are dealing with microbial diversity analysis from different hot springs of Gujarat, India. I am interested to participate workshop of NGS-ION TORRENT and NGS Data analysis by Bioinformatics...

02 July 2014 7,084 3 View

How can I curation a forward and reverse primer sequence?

We have used 27F and 1492R primers for amplification of 16S rRNA gene. We got 1.5 kb amplicon. We sent our PCR amplicon/product to commercial sequencing service. They sent me forward and reverse...

29 June 2014 241 2 View

Can someone advise on the selection of hypervariable region of 16S rRNA for studying microbial diversity?

For comparative analysis of different hot spring for their microbial diversity, we want to use Ion torrent PGM platform and 454 pyrosequencing method. I need suggestion for selection of...

23 June 2014 6,346 4 View

Can someone provide information regarding microbial community analysis from hot spring water using Metageneassist tool?

We are dealing with microbial community analysis from hot spring water using Metageneassist tool. We prepared metadata file and downloaded organism abundance table from MG-RAST and uploaded in...

23 June 2014 8,621 0 View

Which bioinformatic tool is good for comparative analysis of microbial diversity from hot spring water?

See above

21 June 2014 1,041 2 View

Does anyone have an idea regarding the selection of hypervariable regions of 16S rRNA gene for pyrosequencing?

Microbial diversity study.

19 June 2014 2,843 3 View

Can anyone suggest the manual protocol for metagenome isolation from hot spring water ?

I am dealing with metagenome isolation from hot spring water and I want to assess microbial diversity from hot spring water sample.

16 June 2014 8,521 2 View

Regarding analysis of components present in the Urine sample

I have powered form of cattle urine sample. I want to analyse components present in powered urine sample. Which technique (GC-MS, LC-MS, LC-MS-MS, LC-MS QTOF) is most suitable?

01 January 1970 3,416 6 View