We have used 27F and 1492R primers for amplification of 16S rRNA gene. We got 1.5 kb amplicon. We sent our PCR amplicon/product to commercial sequencing service. They sent me forward and reverse primer sequence. Then what should I do to curate both sequences? Which software is effective for multiple sequence alignment, Bioedit or ClustalW? I want to prepare a phylogenetic tree using MEGA5 software. Please help me.