Can anyone suggest the best chromatographic technique to be adopted to well separate high molecular weight tannins?

More Sanmugapriya Ekambaram's questions See All

Is it necessary to do HPLC in addition to LCMS to validate the presence of a phytocompound?

I have analysed set of compounds through LCMS. Is it necessary to do HPLC for the confirmation a single compound which is taken from that LCMS data?

23 October 2022 3,193 4 View

LDA underestimates the lattice constant, while GGA overestimates. Who would synthesis a materials if these approximations set the two extreme limits?

LDA underestimates the lattice constant, while GGA overestimates. Can these two approximations set the two extreme limits for an experimentalist who would synthesize the materials in future?

04 July 2022 2,901 1 View

What is the DFT accuracy limit that would distinguish total energy/cell for two values that are very close to each other?

Hi everyone. Kindly clarify it.

04 July 2022 1,921 2 View

How would your results i.e band gap predictions change if we adopted hybrid functionals instead of GGA functionals?

How band structure will change if we adopt hybrid functional rather GGA.

04 July 2022 5,316 1 View

How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,563 3 View

How to find the dihedral angle of particular residue over the period of simulation time?

Dear Friends, I would like to perform the dihedral angle calculation of particular residue over the period of simulation time. Could you please help me out in creating the ndx file using make_ndx...

19 March 2018 2,462 3 View

Is there any quick way where I can approximately calculate the Peak Power, Intermittent Power of Induction motor, having known the Continuous Power?

I have a 60V, 4.5kW 3-phase AC induction motor. Assuming that the rated power is the continuous power and with no other motor data, can I predict the peak and intermittent power of the motor? I...

14 February 2018 3,622 3 View

How to perform the PIEDA calculation for the receptor-ligand residues using FMO ?

Dear All, Im trying to compute the PIEDA for the receptor-ligand residues using the FMO calculation. Could you help me in providing the steps to create the input put file for running the...

10 November 2017 324 2 View

How to calculate the host-guest binding affinity using quantum mechanics

I'm trying to find out the binding affinity between the host-guest complex using Mopac. I have only the complexed and uncomplexed files. It would be helpful if someone could explain me with the...

16 June 2017 2,097 3 View

Which is the alternate animal model for type II collagen induced arthritis in DBA/1 mice?

Is there any alternate animal model for type II collagen induced arthritis in DBA/1 mice? DBA/1 mice is not available easily and thus suggestion on any preferred animal model for the study is...

19 December 2016 5,171 2 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View