Hi,

You could do a search in pubmed using  Mesh terms. Check NCBI tutorials and HOWTO for details. Image represent a search for the following terms:

( "Vaccination/administration and dosage"[Mesh] OR "Vaccination/immunology"[Mesh] OR "Vaccination/methods"[Mesh] ) AND (recall OR BOOST)

If you are working in clinical immunology than useful key words will be  Immunization dose response, post immunization dose effect, biological efficacy of vaccine, therapeutic effects, individual and cumulative, reversal effects and SIT.

Hi Alekhya,

if you want to monitor T cells, the keyword of interest is "T cell kinetics". You will see that you'll have a sharp expansion after 10-14 days. After that a memory response is established. Once you have a memory response, you will have to restimulate your T cells in order to see effector functions. So indeed, your way of thinking about the right time point is the correct approach before starting the experiment.

Antibody responses form after several weeks. You can search for "IgG titer"  and so on. Here you see that it is difficult to monitor T cells and antibodies on the same time point.

Good luck!

Christoph

Thank you for the suggestions. I am trying to characterize my prime. I did a priming followed by tissue sample collection at Day0(no sham,no immunogen), Day5 and Day42. I wasn't able to see much at either Day5 or Day42 so decided to revise the immunization protocol. I was also wondering if doing a boost is the best way to assess a prime or simply doing a prime is good enough.